On microelectrode ionophoresis.

Chapter 1 Using microelectrodes

Microelectrodes are the basis of the techniques discussed in this book. They are used for: (1) potential recording; (2) current injection; (3) introduction into the cell of ionselective resins for measuring potential or determining the free concentration of cytosolic constituents. (1) and (2) are the procedures underlying conventional microelectrode recording, voltage-clamping and patch-clampin...

Nucleotide 'maps' of digests of deoxyribonucleic acid.

Various digests of (32)P-labelled DNA were examined by two-dimensional ionophoresis on cellulose acetate and DEAE-cellulose paper. The products from digestion with pancreatic deoxyribonuclease and Neurospora crassa endonuclease were qualitatively closely similar, but very complex, and were used to investigate the mapping behaviour of nucleotides in various ionophoretic systems. Ionophoresis on ...

Separation and determination of ribonucleotides and related compounds by ionophoresis on filter paper.

The metabolic turnover of the nucleic acids and their constituent parts has been a subject of increasing significance during recent years. Such studies have been facilitated in this laboratory by the use of ionophoresis on filter paper. The usefulness of this technique is accentuated by the fact that very small amounts of material may be used, that actual physical separation is achieved, and th...

An electrophysiologic study of rabbit ciliary epithelium.

Microelectrode recordings from cells in rabbit ciliary epithelium have been made in vitro. Ionophoresis of Lucifer Yellow dye from microelectrodes during measurements of potential confirmed that the recordings were intracellular. Dye passed from the impaled cells into adjacent cells in both the nonpigmented and pigmented layers of the epithelium. Electrical coupling between epithelial cells als...

Filter-paper ionophoresis of cupric complexes of neutral amino acids and oligopeptides.